Vitamin A analysis is based on extraction; HPLC device is used for the final result. Results are generally given in IU / kg.
In addition to the most basic unit IU / kg; it is possible to use other vitamin units. In this sense, units such as Vitamin A acetate and Vitamin A palmitate are also encountered.
Vitamin A analysis results can be given in either acetate or palmitate. Regardless of the unit of results expressed; the same pretreatment is applied to the sample. The saponified sample is made available to the last device. Calibration curve is used in which vitamin A results are desired and analysis results are obtained. As a second way, the unit conversions can be performed and the results can be converted to the desired Vitamin A result. When separate analyzes for acetate and palmitate are performed on standards; The results of the unit conversion were obtained and controls were made.
The following table shows the most commonly used Vitamin A units and conversion factors.
Table of Vitamin A Units
| Retinol activity equivalent (RAE) and vitamin A activity (IU) conversion factors of the amount of vitamin A source material | ||
| Source Material (1 mcg) | Amount of Vitamin A (mcg RAE) | Vitamin A activity (IU) |
| all-trans beta karoten (beta karoten) | 0.50 | 1.67 |
| all-trans retinol (1 mcg RAE) | 1.00 | 3.33 |
| all-trans retinil asetat (vitamin A asetat) | 0.87 | 2.94 |
| all-trans retinil palmitat (vitamin A palmitat) | 0.55 | 1.82 |
Table Vitamin A Units The retinol activity equivalent (RAE) and vitamin A activity (IU) conversion factors of the amount of vitamin A source material.
Improper preparation of premix for vitamin A analysis
Failure to properly mix premix into flour sample
- Premix and sample storage conditions (Vitamin A is particularly affected by light and air)
© 1994 | Saniter Gıda – Çevre Bilimi Gözetim Ve Mühendislik Hiz. Tur. Tic. A.Ş.
