Vitamin A analysis is based on extraction; HPLC device is used for the final result. Results are generally given in IU / kg.
In addition to the most basic unit IU / kg; it is possible to use other vitamin units. In this sense, units such as Vitamin A acetate and Vitamin A palmitate are also encountered.
Vitamin A analysis results can be given in either acetate or palmitate. Regardless of the unit of results expressed; the same pretreatment is applied to the sample. The saponified sample is made available to the last device. Calibration curve is used in which vitamin A results are desired and analysis results are obtained. As a second way, the unit conversions can be performed and the results can be converted to the desired Vitamin A result. When separate analyzes for acetate and palmitate are performed on standards; The results of the unit conversion were obtained and controls were made.
The following table shows the most commonly used Vitamin A units and conversion factors.
Table of Vitamin A Units
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Retinol activity equivalent (RAE) and vitamin A activity (IU) conversion factors of the amount of vitamin A source material |
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|
Source Material (1 mcg) |
Amount of Vitamin A (mcg RAE) |
Vitamin A activity (IU) |
|
all-trans beta karoten (beta karoten) |
0.50 |
1.67 |
|
all-trans retinol (1 mcg RAE) |
1.00 |
3.33 |
|
all-trans retinil asetat (vitamin A asetat) |
0.87 |
2.94 |
|
all-trans retinil palmitat (vitamin A palmitat) |
0.55 |
1.82 |
Table Vitamin A Units The retinol activity equivalent (RAE) and vitamin A activity (IU) conversion factors of the amount of vitamin A source material.
Improper preparation of premix for vitamin A analysis
Failure to properly mix premix into flour sample
- Premix and sample storage conditions (Vitamin A is particularly affected by light and air)
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